Yihui Song1, Jingjing You1,2, Con Petsoglou1,3,4, Gerard Sutton1,5,3
Purpose: Currently, there are collagen-based implants used for corneal wound healing which requires suturing and some are not transparent. We have previously developed a transparent collagen ink, which has potential to form collagen gel and ﬁlm. This study is to determine the cell compatibil- ity of the collagen gel and ﬁlm generated by the collagen ink.
Method: UV treatment was applied to the bovine collagen I based ink to produce the collagen gel and ﬁlm. Tests on adhesiveness, ﬂexibility and transpar- ency were done. Transformed human corneal epi- thelial cells were seeded on the surface of the collagen gel in a Petri dish. After 7 days, a piece of collagen gel was obtained, which was then ﬁxed, cryosectioned and stained with DAPI to observe the presence of the cells.
Results: Collagen gel is transparent and adhesive to glass slides and Petri dishes. Collagen ﬁlm is ﬂexi- ble able to fold and unfold within water without external force. Cell growth was observed on the gel surface. DAPI results showed that the cells could migrate into the collagen gel after 7 days with some cells reached 0.15 mm deep (50% thickness) of the collagen gel.
Conclusion: The collagen ink developed previously can be transformed into transparent and adhesive collagen gel and ﬁlm are biocompatible. These abili- ties indicate that the product may be used as stroma constructs without the need of suture, and may have high structural integrity allowing cell migration. Further experiments on primary cells and animal tri- als may be needed to conﬁrm its clinical applications.
COMPARISON OF RANIBIZUMAB AND AFLIBERCEPT IN PATIENTS WITH NEOVASCULAR AGE-RELATED MACULAR DEGENERATION TREATED FOLLOWING A ?TREAT AND EXTEND? PROTOCOL: EFFICACY VARIABLES FROM THE PRE-SPECIFIED 12- MONTH INTERIM ANALYSIS OF THE RIVAL STUDY