ABSTRACT NUMBER - 17

A COLLAGEN INK FOR CORNEAL MELTING


Yihui Song1, Jingjing You1,2, Con Petsoglou1,3,4, Gerard Sutton1,5,3

Meeting:  2018 RANZCO


SESSION INFORMATION

Date:      -

Session Title: POSTER ABSTRACT- Cornea

Session Time:      -

Purpose: Currently, there are collagen-based implants used for corneal wound healing which requires suturing and some are not transparent. We have previously developed a transparent collagen ink, which has potential to form collagen gel and film. This study is to determine the cell compatibil- ity of the collagen gel and film generated by the collagen ink.
Method: UV treatment was applied to the bovine collagen I based ink to produce the collagen gel and film. Tests on adhesiveness, flexibility and transpar- ency were done. Transformed human corneal epi- thelial cells were seeded on the surface of the collagen gel in a Petri dish. After 7 days, a piece of collagen gel was obtained, which was then fixed, cryosectioned and stained with DAPI to observe the presence of the cells.
Results: Collagen gel is transparent and adhesive to glass slides and Petri dishes. Collagen film is flexi- ble able to fold and unfold within water without external force. Cell growth was observed on the gel surface. DAPI results showed that the cells could migrate into the collagen gel after 7 days with some cells reached 0.15 mm deep (50% thickness) of the collagen gel.
Conclusion: The collagen ink developed previously can be transformed into transparent and adhesive collagen gel and film are biocompatible. These abili- ties indicate that the product may be used as stroma constructs without the need of suture, and may have high structural integrity allowing cell migration. Further experiments on primary cells and animal tri- als may be needed to confirm its clinical applications.

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