Jeyanth Rose, Aarwin Joshua, Sanita Korah, Thomas Kuriakose, Augustine Thambaiah, Geetha Chacko
Purpose: To evaluate the effect of Placenta derived Mesenchymal Stem Cells (MSC’s) versus Placebo in improving corneal transparency following experi- mental injury in an ex-vivo organ culture model of post- mortem human corneas.
To compare the inﬂuence of MSC’S on the basic his- topathology of the cornea.
To compare the inﬂuence of MSC’s on the Immuno- histochemistry markers of Fibrotic corneal scarring. Methods: Placenta derived MSC’s extracted from the placenta were isolated and expanded in-vitro.
Five pairs of post mortem human corneas equal in grade underwent a superﬁcial keratectomy, 4 mm in diameter and randomly received intrastromal injec- tion of MSC’s or Placebo.
The corneal button was maintained in an organ cul- ture system for 28 days.
Laser light was passed through the corneas mounted on a self-styled modiﬁed artiﬁcial anterior chamber. Image analysis was used to quantify corneal transparency.
H & E staining and Immunohistochemistry was done for Actin, Tenascin and Keratocan
Results: Laser scatter measurements- The difference in the median of Full Width Half Max(FWHM), Max intensity and Red pixel intensity was −104.9,
14.70 and 11.73 respectively, between case and con- trol respectively. All three parameters showed statis- tically signiﬁcant difference (P < 0.05) Histopathology was characterized by a disorderly Bowmans layer and basement membrane in the con- trols as compared to cases. Actin at the injury site- stained 3+ in all controls compared to cases (1+). Conclusion: Placenta derived Mesenchymal Stem Cells can alter evolving corneal scarring into a more favourable outcome with better corneal transparency and less ﬁbrotic corneal scarring.