ABSTRACT NUMBER - S3010

AN OCULAR ADHESIVE FOR CORNEAL WOUND SEALING: AN EX VIVO STUDY


Jackie Tan, Leslie John Ray Foster, Stephanie Louise Watson

Meeting:  2018 RANZCO


SESSION INFORMATION

Date:      -

Session Title: FREE PAPERS: Rapid Fire Presentations – Cornea

Session Time:      -

Purpose: To assess a chitosan-based laser-activated thin-film corneal ‘adhesive’ for sealing full thickness corneal wounds.
Methods: An ex-vivo comparative study of the ‘adhesive’ was conducted on 40 freshly harvested bovine corneas. A standardized, linear full thickness central corneal wound of 2 mm or 4 mm was created with a scalpel blade. The wound was sealed with the irradiated or non-irradiated (NI) adhesive or left unsealed as controls. The study groups; (1) 2 mm wound with NI adhesive (n = 10), (2) 2 mm wound with irradiated adhesive (n = 10), and (3) 4mm wound with irradiated adhesive (n = 10). The control group was a 2mm wound left to ‘self-seal’ (n = 10). The strength of wound closure was compared using a custom burst pressure meter. The primary outcome was the highest fluid pressure recorded prior to wound leak. Results were compared using two- samples t-test and significance level set at 5%.
Results: The mean ( standard deviation) burst pressures for the controls was 31.2 ( 9.7) mmHg, and the study groups were (1) 84.7( 30.6) mmHg,
(2) 228.8 ( 63.7) mmHg, and (3) 205.1 ( 52.7)
mmHg respectively. There were significant differ- ences in the burst pressure between the self-seal group and 2 mm NI adhesive (P < 0.001); 2 mm NI adhesive and 2 mm irradiated adhesive (P < 0.001); and 2 mm irradiated adhesive and 4 mm irradiated adhesive (P < 0.001). Conclusion: The ocular adhesive sealed corneal wounds under high pressure, laser activation further improved sealing and reached a ‘supraphysiologic’ intraocular pressure in the bovine model.

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