ABSTRACT NUMBER - 110

INNATE IMMUNITY IN ENDOPHTHALMITIS: THE ROLE OF PHAGOCYTOSIS AND THE P2X7 RECEPTOR


Andrew Hurley1,2, Rosie Dawkins3,1, Ben Gu4, Xin Huang4, Penelope Allen1,3

Meeting:  2018 RANZCO


SESSION INFORMATION

Date:      -

Session Title: POSTER ABSTRACT- RETINA

Session Time:      -

Purpose: We hypothesise that innate phagocytic function is one determinant of the development of endophthalmitis. This pilot study compares the function of the P2X7 receptor and phagocytosis in patients with acute endophthalmitis with a popula- tion control group.
Methods: Patients presenting to the RVEEH emer- gency department with acute endophthalmitis were offered entry into the study. Informed consent was obtained, and peripheral blood taken. Quantitative measurement of P2X7 receptor function via uptake of ethidium and phagocytic function via uptake of fluorescent latex beads using a validated flow cytometric-method was undertaken at the Florey Institute. Patients will have repeat bloods taken
3 months from admission to observe phagocytic function through convalescence.
Results: The subjects (n = 8) all had reduced ethi- dium uptake and latex bead phagocytosis compared to controls. Additionally, phagocytic function was not improved with CPX which typically boosts phagocytic function significantly in healthy patients. This was true for all 3 subsets of CD14+ monocytes tested.
Conclusions: This pilot study shows reduced P2X7 receptor function and phagocytic function in acute endophthalmitis. We intend to recruit a larger cohort to elucidate this interesting finding. This may allow us to predict susceptibility or guide future therapeu- tics through enhancement of innate immune function.

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