Jack Ao, Glyn Chidlow, John Wood, Robert Casson
Purpose: Photobiomodulation (PBM) refers to irra- diation of tissue with low energy red/near-infrared light. The convergence of evidence indicates that PBM increases energy production and reduces oxi- dative stress in the mitochondria. We used a novel slit lamp-delivered 670 nm laser to investigate whether PBM augments cone survival in the rd1 mouse model of retinitis pigmentosa.
Method: An initial cohort of rd1 mice received sham (n = 10), 25 mW/cm2 PBM (n = 10), or 100 mW/cm2 PBM (n = 11) twice weekly from P21 to P60 in one eye. The fellow eye was untreated (n = 31). A second cohort received PBM treatment with 100 mW/cm2 to one eye (n = 8) with the fel- low eye serving as an untreated control (n = 8) from P21 to P90. Optokinetic (OKN) testing was per- formed at P35 for all eyes in both cohorts. Cone den- sity was quantiﬁed on wholemount retinas using immunohistochemistry of s-opsin and M/L-opsin at P60 or P90. Statistical analyses were performed with ANOVA or paired t-test.
Results: Mice in the 25 mW/cm2 PBM and 100 mW/cm2 PBM groups had signiﬁcantly higher s- opsin and M/L-opsin counts (P < 0.001) compared to control and sham groups at P60 and P90. PBM also signiﬁcantly (P < 0.001) improved optomotor function in Rd1 mice compared to control and sham groups. Conclusion: PBM signiﬁcantly augmented cone sur- vival up to P90 and improved visual function in rd1 mice. Our study provides the foundation for PBM as a potentially novel treatment for retinitis pigmentosa.
COMPARISON OF RANIBIZUMAB AND AFLIBERCEPT IN PATIENTS WITH NEOVASCULAR AGE-RELATED MACULAR DEGENERATION TREATED FOLLOWING A ?TREAT AND EXTEND? PROTOCOL: EFFICACY VARIABLES FROM THE PRE-SPECIFIED 12- MONTH INTERIM ANALYSIS OF THE RIVAL STUDY