ABSTRACT NUMBER - 115

NEUROPROTECTION EFFICACY OF LASER-DELIVERED PHOTOBIOMODULATION IN A SEVERE MOUSE MODEL OF RETINITIS PIGMENTOSA


Jack Ao, Glyn Chidlow, John Wood, Robert Casson

Meeting:  2018 RANZCO


SESSION INFORMATION

Date:      -

Session Title: POSTER ABSTRACT- RETINA

Session Time:      -

Purpose: Photobiomodulation (PBM) refers to irra- diation of tissue with low energy red/near-infrared light. The convergence of evidence indicates that PBM increases energy production and reduces oxi- dative stress in the mitochondria. We used a novel slit lamp-delivered 670 nm laser to investigate whether PBM augments cone survival in the rd1 mouse model of retinitis pigmentosa.
Method: An initial cohort of rd1 mice received sham (n = 10), 25 mW/cm2 PBM (n = 10), or 100 mW/cm2 PBM (n = 11) twice weekly from P21 to P60 in one eye. The fellow eye was untreated (n = 31). A second cohort received PBM treatment with 100 mW/cm2 to one eye (n = 8) with the fel- low eye serving as an untreated control (n = 8) from P21 to P90. Optokinetic (OKN) testing was per- formed at P35 for all eyes in both cohorts. Cone den- sity was quantified on wholemount retinas using immunohistochemistry of s-opsin and M/L-opsin at P60 or P90. Statistical analyses were performed with ANOVA or paired t-test.
Results: Mice in the 25 mW/cm2 PBM and 100 mW/cm2 PBM groups had significantly higher s- opsin and M/L-opsin counts (P < 0.001) compared to control and sham groups at P60 and P90. PBM also significantly (P < 0.001) improved optomotor function in Rd1 mice compared to control and sham groups. Conclusion: PBM significantly augmented cone sur- vival up to P90 and improved visual function in rd1 mice. Our study provides the foundation for PBM as a potentially novel treatment for retinitis pigmentosa.

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